Our Research Methods Behind MIRA1a Effectiveness
Explanation of Elevated Plus Maze
The Elevated Plus Maze (EPM) is a widely used preclinical behavioral assay for rodents and it has been validated to assess the anti-anxiety effects of pharmacological agents.
The EPM is a test measuring anxiety in rodents as a screening test for putative anxiolytic compounds and a general research tool in neurobiological anxiety research such as GAD or PTSD. The model is based on the test animal’s aversion to open spaces in the maze's open arms.
Anti-anxiety effects of test agents are demonstrated by an increase in the percentage of time spent in the open arm with treatment compared to placebo. The total distance traveled is a measure of the overall level of arousal and mobility of the mice undergoing testing on the EPM, and is used to rule out any sedating or intoxicating effects of the test agent.
Explanation of Hot Plate Test
A number of clinically approved pharmacological agents to treat pain have been demonstrated to delay the onset of heat sensitivity upon paw exposure in mice to heat including opioids.